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pcdna3 1 zeo expression vector  (Thermo Fisher)


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    Thermo Fisher pcdna3 1 zeo expression vector
    Pcdna3 1 Zeo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+zeo+expression+vector/Zeocin/pm41390560-150-188-192
    Average 99 stars, based on 1 article reviews
    pcdna3 1 zeo expression vector - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Primary SARS-CoV-2 exposure by vaccination or infection shapes immune responses to omicron variants among a Spanish cohort.
    Article Snippet: We measured IgA and IgG plasma levels (median fluorescence intensity, MFI) to the fulllength (FL) SARS-CoV-2 nucleocapsid (N) (amino acids 1-416); spike (S) antigens (amino acids 1-1213, amino acid sequence starting MFIF and ending IKWP), and the receptor binding domain (RBD) that lies within the S1 region (RBD ancestral, amino acids 319-541, amino acid sequence starting RVQP and ending CVNF; and variants Delta, BA.1, BA.2, BA.4/5, BQ.1.1, and XBB), by quantitative suspension array technology assays (xMAP, Luminex), following a previously described protocol.34–36 A nucleotide fragment encoding the ancestral N FL, AR TI CL E IN P RE SS ARTICLE IN PRESS followed by a 6xHis-tag, was cloned into pET22b expression vector, transformed in E. coli BL21 DE3, induced with IPTG, and purified by affinity chromatography using HisTrap columns, and controlled for purity by SDS-PAGE and Coomassie staining.37 The ancestral S FL and the RBD proteins were fused with C-terminal 6xHis and StrepTag sequences and purified from the supernatant of lentiviral-transduced CHO-S cells cultured under a fed-batch system.35 Codon-optimized nucleotide fragments encoding the RBD from variants (Delta, BA.1, BA.2, BA.4/5, BQ.1.1, and XBB) were synthesized and cloned into a pcDNA3.1/Zeo (+) expression vector (Thermo Fisher Scientific).38 Recombinant proteins were produced by transient transfection of exponentially growing Freestyle TM 293-F suspension cells (Thermo Fisher Scientific) using the polyethyleneimine (PEI)-precipitation method.

    Synthesized:

    Article Title: Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2
    Article Snippet: .. Codon-optimized nucleotide fragments encoding FcγRIA (P12314, 16-292 residues), FcγRIIA (P12318, 34-217 residues), FcγRIIB (P31994, 43-217 residues) and FcγRIIIA (P08637, 17-208 residues) ectodomains preceded by the human IgK leader peptide and followed C-terminal tags (Hisx8-tag and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). ..

    Clone Assay:

    Article Title: Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2
    Article Snippet: .. Codon-optimized nucleotide fragments encoding FcγRIA (P12314, 16-292 residues), FcγRIIA (P12318, 34-217 residues), FcγRIIB (P31994, 43-217 residues) and FcγRIIIA (P08637, 17-208 residues) ectodomains preceded by the human IgK leader peptide and followed C-terminal tags (Hisx8-tag and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Article Title: Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
    Article Snippet: Plasmid constructs and transient transfection in HeLa cells Human PSMB8, PSMA5, and PSMB10 were amplified by PCR from preexisting pIVEX2.3-based constructs and subcloned into the pcDNA3.1/V5-HIS TOPO expression vector (Thermo Fisher Scientific). .. Human PSMC5 was PCR-amplified from cDNA generated from HeLa cells with HA-tagged primers and cloned into pcDNA3.1/Zeo(+) expression vector (Invitrogen) to generate a N-terminally Hemagglutinin (HA)-tagged PSMC5/Rpt6 protein. ..

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: Expression Plasmids. cDNA encoding a C-terminal fusion of the WT human RSV-F ectodomain or DS-Cav1protein ectodomain to the T4 fibritin foldon trimerization motif, thrombin cleavage-site, 6× HIS-tag (SEQ ID NO: 46), and strep-tag were codon-optimized for human expression and synthesized (Geneart). cDNA encoding RSV F protein variants E222Y (SEQ ID NO:13), K226Y (SEQ ID NO:15), V469Y (SEQ ID NO:16), N88Y/S255Y (SEQ ID NO:18), V185Y/K427Y (SEQ ID NO:20) were also synthesized. .. These DNA sequences were cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (FIG. 33). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Expressing:

    Article Title: Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2
    Article Snippet: .. Codon-optimized nucleotide fragments encoding FcγRIA (P12314, 16-292 residues), FcγRIIA (P12318, 34-217 residues), FcγRIIB (P31994, 43-217 residues) and FcγRIIIA (P08637, 17-208 residues) ectodomains preceded by the human IgK leader peptide and followed C-terminal tags (Hisx8-tag and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Article Title: Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
    Article Snippet: Plasmid constructs and transient transfection in HeLa cells Human PSMB8, PSMA5, and PSMB10 were amplified by PCR from preexisting pIVEX2.3-based constructs and subcloned into the pcDNA3.1/V5-HIS TOPO expression vector (Thermo Fisher Scientific). .. Human PSMC5 was PCR-amplified from cDNA generated from HeLa cells with HA-tagged primers and cloned into pcDNA3.1/Zeo(+) expression vector (Invitrogen) to generate a N-terminally Hemagglutinin (HA)-tagged PSMC5/Rpt6 protein. ..

    Article Title: Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates
    Article Snippet: .. These were inserted into the pcDNA3.1-Zeo expression vector (Invitrogen, San Diego, CA) driven by a CMV viral promoter. ..

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: Expression Plasmids. cDNA encoding a C-terminal fusion of the WT human RSV-F ectodomain or DS-Cav1protein ectodomain to the T4 fibritin foldon trimerization motif, thrombin cleavage-site, 6× HIS-tag (SEQ ID NO: 46), and strep-tag were codon-optimized for human expression and synthesized (Geneart). cDNA encoding RSV F protein variants E222Y (SEQ ID NO:13), K226Y (SEQ ID NO:15), V469Y (SEQ ID NO:16), N88Y/S255Y (SEQ ID NO:18), V185Y/K427Y (SEQ ID NO:20) were also synthesized. .. These DNA sequences were cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (FIG. 33). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Plasmid Preparation:

    Article Title: Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2
    Article Snippet: .. Codon-optimized nucleotide fragments encoding FcγRIA (P12314, 16-292 residues), FcγRIIA (P12318, 34-217 residues), FcγRIIB (P31994, 43-217 residues) and FcγRIIIA (P08637, 17-208 residues) ectodomains preceded by the human IgK leader peptide and followed C-terminal tags (Hisx8-tag and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Article Title: Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
    Article Snippet: Plasmid constructs and transient transfection in HeLa cells Human PSMB8, PSMA5, and PSMB10 were amplified by PCR from preexisting pIVEX2.3-based constructs and subcloned into the pcDNA3.1/V5-HIS TOPO expression vector (Thermo Fisher Scientific). .. Human PSMC5 was PCR-amplified from cDNA generated from HeLa cells with HA-tagged primers and cloned into pcDNA3.1/Zeo(+) expression vector (Invitrogen) to generate a N-terminally Hemagglutinin (HA)-tagged PSMC5/Rpt6 protein. ..

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: Expression Plasmids. cDNA encoding a C-terminal fusion of the WT human RSV-F ectodomain or DS-Cav1protein ectodomain to the T4 fibritin foldon trimerization motif, thrombin cleavage-site, 6× HIS-tag (SEQ ID NO: 46), and strep-tag were codon-optimized for human expression and synthesized (Geneart). cDNA encoding RSV F protein variants E222Y (SEQ ID NO:13), K226Y (SEQ ID NO:15), V469Y (SEQ ID NO:16), N88Y/S255Y (SEQ ID NO:18), V185Y/K427Y (SEQ ID NO:20) were also synthesized. .. These DNA sequences were cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (FIG. 33). ..

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Mutagenesis:

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Modification:

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Article Title: Conformationally stabilized RSV pre-fusion F proteins
    Article Snippet: .. Mutant RSV F proteins were expressed in human cells and modified by the introduction of di-tyrosine bonds as described below. cDNA encoding mutant RSV F molecules was cloned into the pCDNA3.1/zeo+ expression vector (Invitrogen) via 5′ BamHI and 3′XhoI restriction endonuclease sites using standard methods (See FIG. 1). .. HEK 293 cells (ATCC) were grown in Dulbecco's Modification of Eagle's Medium (DMEM, Invitrogen) supplemented with 10% Fetal Bovine Serum and 50 μg/ml gentamycin.

    Polymerase Chain Reaction:

    Article Title: Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
    Article Snippet: Plasmid constructs and transient transfection in HeLa cells Human PSMB8, PSMA5, and PSMB10 were amplified by PCR from preexisting pIVEX2.3-based constructs and subcloned into the pcDNA3.1/V5-HIS TOPO expression vector (Thermo Fisher Scientific). .. Human PSMC5 was PCR-amplified from cDNA generated from HeLa cells with HA-tagged primers and cloned into pcDNA3.1/Zeo(+) expression vector (Invitrogen) to generate a N-terminally Hemagglutinin (HA)-tagged PSMC5/Rpt6 protein. ..

    Generated:

    Article Title: Identification of eight novel proteasome variants in five unrelated cases of proteasome-associated autoinflammatory syndromes (PRAAS).
    Article Snippet: Plasmid constructs and transient transfection in HeLa cells Human PSMB8, PSMA5, and PSMB10 were amplified by PCR from preexisting pIVEX2.3-based constructs and subcloned into the pcDNA3.1/V5-HIS TOPO expression vector (Thermo Fisher Scientific). .. Human PSMC5 was PCR-amplified from cDNA generated from HeLa cells with HA-tagged primers and cloned into pcDNA3.1/Zeo(+) expression vector (Invitrogen) to generate a N-terminally Hemagglutinin (HA)-tagged PSMC5/Rpt6 protein. ..

    Recombinant:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Produced:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Transfection:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Suspension:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Purification:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..

    Chromatography:

    Article Title: High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination.
    Article Snippet: .. Codon-optimized nucleotide fragments encoding the RBD Omicron subvariants were cloned into a pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific), recombinant proteins were produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher Scientific) and subsequently purified from supernatant by high-performance chromatography, as previously described [17]. ..



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